| First Author | Wu YW | Year | 2025 |
| Journal | Dev Cell | Volume | 60 |
| Issue | 1 | Pages | 85-100.e4 |
| PubMed ID | 39378876 | Mgi Jnum | J:360942 |
| Mgi Id | MGI:7779468 | Doi | 10.1016/j.devcel.2024.09.009 |
| Citation | Wu YW, et al. (2024) RNA surveillance by the RNA helicase MTR4 determines volume of mouse oocytes. Dev Cell |
| abstractText | Oocytes are the largest cell type in multicellular animals. Here, we show that mRNA transporter 4 (MTR4) is indispensable for oocyte growth and functions as part of the RNA surveillance mechanism, which is responsible for nuclear waste RNA clearance. MTR4 ensures the normal post-transcriptional processing of maternal RNAs, their nuclear export to the cytoplasm, and the accumulation of properly processed transcripts. Oocytes with Mtr4 knockout fail to accumulate sufficient and normal transcripts in the cytoplasm and cannot grow to normal sizes. MTR4-dependent RNA surveillance has a previously unrecognized function in maintaining a stable nuclear environment for the establishment of non-canonical histone H3 lysine-4 trimethylation and chromatin reorganization, which is necessary to form a nucleolus-like structure in oocytes. In conclusion, MTR4-dependent RNA surveillance activity is a checkpoint that allows oocytes to grow to a normal size, undergo nuclear and cytoplasmic maturation, and acquire developmental competence. |
