| First Author | Fayer S | Year | 2016 |
| Journal | Mamm Genome | Volume | 27 |
| Issue | 5-6 | Pages | 225-36 |
| PubMed ID | 27090237 | Mgi Jnum | J:242431 |
| Mgi Id | MGI:5905229 | Doi | 10.1007/s00335-016-9630-2 |
| Citation | Fayer S, et al. (2016) Robertsonian translocations modify genomic distribution of gammaH2AFX and H3.3 in mouse germ cells. Mamm Genome 27(5-6):225-36 |
| abstractText | Heterozygosity for Robertsonian translocations hampers pairing and synapsis between the translocated chromosome and its normal homologs during meiotic prophase I. This causes meiotic silencing of unsynapsed chromatin in pericentromeric regions. Several lines of evidence suggest that autosomal asynapsis leads to meiotic arrest in males and two underlying mechanisms have been proposed: (1) reactivation of the X and Y chromosomes due to competition for silencing factors and (2) meiotic silencing of genes that are located in the unsynapsed regions and are essential for meiotic progression. The latter mechanism requires that asynapsis and meiotic silencing spread beyond the p-arms of the normal homologs into gene-rich regions. We used chromatin immunoprecipitation assays to determine whether histones gammaH2AFX and H3.3, both marks of asynapsis and meiotic silencing, are enriched in gene-rich regions of the translocated chromosomes and their homologs in the spermatocytes of heterozygous carriers of Robertsonian translocations. We also asked if gammaH2AFX and H3.3 enrichment was reduced at the X chromosome and if gammaH2AFX and H3.3 enrichment was higher on the normal homolog. Our data show that gammaH2AFX enrichment extends as far as 9-15 Mb of the annotated genomic sequence of the q-arms of the translocated chromosomal trivalents and that both gammaH2AFX and H3.3 levels are reduced over the X chromosome. Our data are also suggestive of an asymmetry in gammaH2AFX and H3.3 enrichment with a bias toward the non-translocated homolog. |
