| First Author | Jang MK | Year | 2017 |
| Journal | PLoS One | Volume | 12 |
| Issue | 1 | Pages | e0168185 |
| PubMed ID | 28060835 | Mgi Jnum | J:246120 |
| Mgi Id | MGI:5916507 | Doi | 10.1371/journal.pone.0168185 |
| Citation | Jang MK, et al. (2017) Histone H3K9 Demethylase JMJD2B Activates Adipogenesis by Regulating H3K9 Methylation on PPARgamma and C/EBPalpha during Adipogenesis. PLoS One 12(1):e0168185 |
| abstractText | Previous studies have shown that tri- or di-methylation of histone H3 at lysine 9 (H3K9me3/me2) on the promoter of the peroxisome proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein alpha (C/EBPalpha) contribute to the repression of PPARgamma and C/EBPalpha and inhibition of adipogenesis in 3T3-L1 preadipocytes. The balance of histone methylation is regulated by histone methyltransferases and demethylases. However, it is poorly understood which demethylases are responsible for removing H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha. JMJD2B is a H3K9me3/me2 demethylase that was previously shown to activate adipogenesis by promoting mitotic clonal expansion. Nevertheless, it remains unclear whether JMJD2B plays a role in the regulation of adipogenesis by removing H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha and subsequently activating PPARgamma and C/EBPalpha expression. Here, we showed that JMJD2B decreased H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha, which in turn stimulated the expression of PPARgamma and C/EBPalpha. JMJD2B knockdown using siRNA in 3T3-L1 preadipocytes repressed the expression of PPARgamma and C/EBPalpha, resulting in inhibition of adipogenesis. This was accompanied by increased enrichment of H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha. In contrast, overexpression of JMJD2B increased the expression of PPARgamma and C/EBPalpha, which was accompanied by decreased enrichment of H3K9me3/me2 on the promoter and activated adipogenesis. Together, these results indicate that JMJD2B regulates PPARgamma and C/EBPalpha during adipogenesis. |
