| First Author | Mori D | Year | 2017 |
| Journal | PLoS One | Volume | 12 |
| Issue | 1 | Pages | e0169562 |
| PubMed ID | 28046067 | Mgi Jnum | J:246907 |
| Mgi Id | MGI:5918962 | Doi | 10.1371/journal.pone.0169562 |
| Citation | Mori D, et al. (2017) C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein beta-Glucuronidase on Dendritic Cells. PLoS One 12(1):e0169562 |
| abstractText | C-type lectin receptors (CLRs) recognize pathogen-derived ligands and abnormal self that trigger protective immune responses. However, the precise nature of self ligands recognized by CLRs remains to be determined. Here, we found that Dectin-2 recognizes bone marrow-derived dendritic cells (BMDCs) using Dectin-2-expressing reporter cells. This activity was inhibited by an excessive amount of mannose, and by the mutation of mannose-binding motif in Dectin-2. beta-glucuronidase (Gusb) was identified as a protein bound to Dectin-2 and mutations of N-glycosylation sites in Gusb impaired the binding of Gusb to Dectin-2. Overexpression of Gusb in a macrophage cell line conferred an ability to stimulate Dectin-2-expressing reporter cells. Our study suggests that a glycosylated protein with mannose-related structure is recognized by Dectin-2. |
