| First Author | Suzuki-Yamazaki N | Year | 2017 |
| Journal | Eur J Immunol | Volume | 47 |
| Issue | 3 | Pages | 493-503 |
| PubMed ID | 28012163 | Mgi Jnum | J:246411 |
| Mgi Id | MGI:5924471 | Doi | 10.1002/eji.201646549 |
| Citation | Suzuki-Yamazaki N, et al. (2017) IL-10 production in murine IgM+ CD138hi cells is driven by Blimp-1 and downregulated in class-switched cells. Eur J Immunol 47(3):493-503 |
| abstractText | In contrast to antibody-induced inflammatory responses, some B-cell subpopulations suppress inflammation through the production of interleukin (IL)-10. However, the mechanisms underlying Il10 gene expression during B-cell development is elusive. Here, we identify IgM+ B220lo CD138hi cells responsible for marked IL-10 production in the bone marrow and spleen of mice. These murine IL-10-producing cells predominantly secrete IgM and have unique characteristics of long-lived plasma cells in spite of high expression of surface IgM. We found that IL-10 production is strongly correlated with the expression level of Prdm1 (encoding the Blimp-1 protein), an essential regulator of plasma cell development. Furthermore, overexpression of Prdm1 induces Il10 expression in naive B cells. Immunoglobulin class-switching recombination events resulted in the downregulation of both Il10 and Prdm1 expression in differentiating B cells. Thus, the prolonged elevation of Blimp-1 expression during the formation of IgM+ CD138hi cells without class-switching elicits IL-10 production. Adoptive transfer of Il10-deficient B cells into B-cell-deficient mice demonstrated that IgM+ CD138hi cell-derived IL-10 supports the survival of class-switched plasma cells and their antibody production in response to antigen challenge. These findings reveal an important role for IL-10 secretion by IgM+ CD138hi cells in the complete and efficient humoral response. |
