| First Author | Dolgacheva LP | Year | 2016 |
| Journal | Arch Biochem Biophys | Volume | 593 |
| Pages | 38-49 | PubMed ID | 26850364 |
| Mgi Jnum | J:252275 | Mgi Id | MGI:6093237 |
| Doi | 10.1016/j.abb.2016.02.001 | Citation | Dolgacheva LP, et al. (2016) Angiotensin II activates different calcium signaling pathways in adipocytes. Arch Biochem Biophys 593:38-49 |
| abstractText | Angiotensin II (Ang II) is an important mammalian neurohormone involved in reninangiotensin system. Ang II is produced both constitutively and locally by RAS systems, including white fat adipocytes. The influence of Ang II on adipocytes is complex, affecting different systems of signal transduction from early capital ES, Cyrillicsmall a, Cyrillic(2+) responses to cell proliferation and differentiation, triglyceride accumulation, expression of adipokine-encoding genes and adipokine secretion. It is known that white fat adipocytes express all RAS components and Ang II receptors (capital A, Cyrilliccapital TE, Cyrillic1 and capital A, Cyrilliccapital TE, Cyrillic2). The current work was carried out with the primary white adipocytes culture, and capital ES, Cyrillicsmall a, Cyrillic(2+) signaling pathways activated by Ang II were investigated using fluorescent microscopy. capital ES, Cyrillicsmall a, Cyrillic(2+)-oscillations and transient responses of differentiated adipocytes to Ang II were registered in cells with both small and multiple lipid inclusions. Using inhibitory analysis and selective antagonists, we now show that Ang II initiates periodic capital ES, Cyrillicsmall a, Cyrillic(2+)-oscillations and transient responses by activating capital A, Cyrilliccapital TE, Cyrillic1 and capital A, Cyrilliccapital TE, Cyrillic2 receptors and involving branched signaling cascades: 1) Ang II --> Gq --> PLC --> IP3 --> IP3Rs --> Ca(2+) 2) Gbetagamma --> PI3Kgamma --> PKB 3) PKB --> eNOS --> NO --> PKG 4) CD38 --> cADPR --> RyRs --> Ca(2+) In these cascades, AT1 receptors play the leading role. The results of the present work open a perspective of using Ang II for correction of signal resistance of adipocytes often observed during obesity and type 2 diabetes. |
