| First Author | Laverrière JN | Year | 2016 |
| Journal | Mol Cell Endocrinol | Volume | 434 |
| Pages | 250-65 | PubMed ID | 27402603 |
| Mgi Jnum | J:256905 | Mgi Id | MGI:6095596 |
| Doi | 10.1016/j.mce.2016.07.010 | Citation | Laverriere JN, et al. (2016) Epigenetic regulation of alternative promoters and enhancers in progenitor, immature, and mature gonadotrope cell lines. Mol Cell Endocrinol 434:250-65 |
| abstractText | Gonadotrope cell identity genes emerge in a stepwise process during mouse pituitary development. Cga, encoding for the alpha-subunit of TSH, LH, and FSH, is initially detected at E11.5 followed by Gnrhr and steroidogenic factor Sf1 at E13.5, specifying cells engaged in a gonadotrope cell fate. Lhb and Fshb appear at E16.5 and 17.5, respectively, typifying differentiated gonadotrope cells. Using the alphaT1-1, alphaT3-1 and LbetaT2 cell lines recapitulating these stages of gonadotrope differentiation, DNA methylation at Gnrhr and Sf1 was investigated. Regulatory regions were found hypermethylated in progenitor alphaT1-1 cells and hypomethylated in differentiated LbetaT2 cells. Abundance of RNA polymerase II together with active histone modifications including H3K4me1, H3K4me3, and H3K27ac were strictly correlated with DNA hypomethylation. Analyses of epigenomic modifications and chromatin accessibility were further extended to Isl1, Lhx3, Gata2, and Pitx2, highlighting alternative usages of specific regulatory gene domains in progenitor alphaT1-1, immature alphaT3-1, and mature LbetaT2 gonadotrope cells. |
