| First Author | Schindler SE | Year | 2015 |
| Journal | Sci Rep | Volume | 5 |
| Pages | 13627 | PubMed ID | 26350769 |
| Mgi Jnum | J:263574 | Mgi Id | MGI:6102620 |
| Doi | 10.1038/srep13627 | Citation | Schindler SE, et al. (2015) Photo-activatable Cre recombinase regulates gene expression in vivo. Sci Rep 5:13627 |
| abstractText | Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a photo-activatable Cre recombinase (PA-Cre) to stably modify gene expression in the mouse brain. Blue light illumination for 12 hours via optical fibers activated PA-Cre in the hippocampus, a deep brain structure. Two-photon illumination through a thinned skull window for 100 minutes activated PA-Cre within a sub-millimeter region of cortex. Light activation of PA-Cre may allow permanent gene modification with improved spatiotemporal precision compared to standard methods. |
