| First Author | Pan M | Year | 2017 |
| Journal | Biochem Biophys Res Commun | Volume | 490 |
| Issue | 2 | Pages | 356-363 |
| PubMed ID | 28647374 | Mgi Jnum | J:251108 |
| Mgi Id | MGI:6103283 | Doi | 10.1016/j.bbrc.2017.06.048 |
| Citation | Pan M, et al. (2017) Overexpression of Dentin matrix protein 1 in Nestin(+) cells causes bone loss in mouse long bone. Biochem Biophys Res Commun 490(2):356-363 |
| abstractText | The well-known matrix protein Dentin matrix protein 1 (DMP1) is expressed by osteoblasts and osteocytes in bone, and it controls bone mineralization. Recently, it has been found that DMP1 is also expressed in other cell types, such as chondrocytes. Nestin(+) cells are one important type of progenitor cell in bone marrow and are associated with bone remodeling. In our preliminary experiment, DMP1 could also be detected in Nestin(+) cells in bone marrow. This study was designed to explore the effect on bone of DMP1 in Nestin(+) cells. A transgenic mouse model with DMP1 expression driven by the Nestin promoter was generated. In vivo and in vitro experiments revealed that overexpression of DMP1 in Nestin(+) cells could limit the proliferation and osteogenic differentiation of BMMSCs, subsequently leading to decreased bone mass. Lower expression of bone matrix protein and a lower bone deposition rate were also observed. Meanwhile, overexpression of DMP1 in Nestin(+) cells had no influence on osteoclast activity. These data indicate that DMP1 plays negative roles in differentiation of Nestin(+) cells and bone formation. |
