| First Author | Lisi V | Year | 2017 |
| Journal | Cell Rep | Volume | 20 |
| Issue | 5 | Pages | 1136-1147 |
| PubMed ID | 28768198 | Mgi Jnum | J:254243 |
| Mgi Id | MGI:6104166 | Doi | 10.1016/j.celrep.2017.07.010 |
| Citation | Lisi V, et al. (2017) Enhanced Neuronal Regeneration in the CAST/Ei Mouse Strain Is Linked to Expression of Differentiation Markers after Injury. Cell Rep 20(5):1136-1147 |
| abstractText | Peripheral nerve regeneration after injury requires a broad program of transcriptional changes. We investigated the basis for the enhanced nerve regenerative capacity of the CAST/Ei mouse strain relative to C57BL/6 mice. RNA sequencing of dorsal root ganglia (DRG) showed a CAST/Ei-specific upregulation of Ascl1 after injury. Ascl1 overexpression in DRG neurons of C57BL/6 mice enhanced their neurite outgrowth. Ascl1 is regulated by miR-7048-3p, which is downregulated in CAST/Ei mice. Inhibition of miR-7048-3p enhances neurite outgrowth. Following injury, CAST/Ei neurons largely retained their mature neuronal profile as determined by single-cell RNA- seq, whereas the C57BL/6 neurons acquired an immature profile. These findings suggest that one facet of the enhanced regenerative phenotype is preservation of neuronal identity in response to injury. |
