| First Author | Dor-On E | Year | 2017 |
| Journal | Sci Signal | Volume | 10 |
| Issue | 481 | PubMed ID | 28559444 |
| Mgi Jnum | J:258610 | Mgi Id | MGI:6142154 |
| Doi | 10.1126/scisignal.aal3154 | Citation | Dor-On E, et al. (2017) T-plastin is essential for basement membrane assembly and epidermal morphogenesis. Sci Signal 10(481) |
| abstractText | The establishment of epithelial architecture is a complex process involving cross-talk between cells and the basement membrane. Basement membrane assembly requires integrin activity but the role of the associated actomyosin cytoskeleton is poorly understood. Here, we identify the actin-bundling protein T-plastin (Pls3) as a regulator of basement membrane assembly and epidermal morphogenesis. In utero depletion of Pls3 transcripts in mouse embryos caused basement membrane and polarity defects in the epidermis but had little effect on cell adhesion and differentiation. Loss-of-function experiments demonstrated that the apicobasal polarity defects were secondary to the disruption of the basement membrane. However, the basement membrane itself was profoundly sensitive to subtle perturbations in the actin cytoskeleton. We further show that Pls3 localized to the cell cortex, where it was essential for the localization and activation of myosin II. Inhibition of myosin II motor activity disrupted basement membrane organization. Our results provide insights into the regulation of cortical actomyosin and its importance for basement membrane assembly and skin morphogenesis. |
