| First Author | Perdikari A | Year | 2017 |
| Journal | Sci Signal | Volume | 10 |
| Issue | 466 | PubMed ID | 28196906 |
| Mgi Jnum | J:259328 | Mgi Id | MGI:6142219 |
| Doi | 10.1126/scisignal.aaf5357 | Citation | Perdikari A, et al. (2017) A high-throughput, image-based screen to identify kinases involved in brown adipocyte development. Sci Signal 10(466) |
| abstractText | Brown adipose tissue (BAT) is responsible for thermogenesis that is not associated with shivering through the process of converting chemical energy into heat through uncoupling protein 1 (UCP1) in the mitochondria. Thus, expanding or activating BAT could be a potential tool against obesity. To analyze the effect of kinase signaling on brown adipocyte formation, a process that describes the acquisition of the ability to dissipate energy as heat, we performed lentiviral-mediated short hairpin knockdown or used pharmacological inhibitors in a high-content and high-throughput in vitro image-based screen. We identified 190 kinases that either stimulated or inhibited brown adipocyte proliferation, differentiation, or formation. Among these kinases, we found that 5'' AMP-activated protein kinase (AMPK) promoted the formation of brown adipocytes abundant inUCP1. Together, our results provide insight into the kinases, particularly AMPK, that regulate brown adipocyte formation. |
