| First Author | El Hachmane MF | Year | 2018 |
| Journal | Biochem J | Volume | 475 |
| Issue | 3 | Pages | 691-704 |
| PubMed ID | 29335300 | Mgi Jnum | J:258236 |
| Mgi Id | MGI:6120333 | Doi | 10.1042/BCJ20170484 |
| Citation | El Hachmane MF, et al. (2018) Extracellular ATP activates store-operated Ca(2+) entry in white adipocytes: functional evidence for STIM1 and ORAI1. Biochem J 475(3):691-704 |
| abstractText | In the present study, we have applied ratiometric measurements of intracellular Ca(2+) concentrations ([Ca(2+)]i) to show that extracellularly applied ATP (adenosine triphosphate) (100 microM) stimulates store-operated Ca(2+) entry (SOCE) in 3T3-L1 adipocytes. ATP produced a rapid increase in [Ca(2+)]i consisting of an initial transient elevation followed by a sustained elevated phase that could be observed only in the presence of extracellular Ca(2+) Gene expression data and [Ca(2+)]i recordings with uridine-5''-triphosphate or with the phospholipase C (PLC) inhibitor U73122 demonstrated the involvement of purinergic P2Y2 receptors and the PLC/inositol trisphosphate pathway. The [Ca(2+)]i elevation produced by reintroduction of a Ca(2+)-containing intracellular solution to adipocytes exposed to ATP in the absence of Ca(2+) was diminished by known SOCE antagonists. The chief molecular components of SOCE, the stromal interaction molecule 1 (STIM1) and the calcium release-activated calcium channel protein 1 (ORAI1), were detected at the mRNA and protein level. Moreover, SOCE was largely diminished in cells where STIM1 and/or ORAI1 had been silenced by small interfering (si)RNA. We conclude that extracellular ATP activates SOCE in white adipocytes, an effect predominantly mediated by STIM1 and ORAI1. |
