| First Author | Kitahashi M | Year | 2007 |
| Journal | DNA Cell Biol | Volume | 26 |
| Issue | 6 | Pages | 395-401 |
| PubMed ID | 17570763 | Mgi Jnum | J:259301 |
| Mgi Id | MGI:6149130 | Doi | 10.1089/dna.2006.0565 |
| Citation | Kitahashi M, et al. (2007) Identification of the consensus DNA sequence for Nczf binding. DNA Cell Biol 26(6):395-401 |
| abstractText | The Nczf gene, which is identified as a target gene of Ncx, encodes a novel Kruppel-associated box (KRAB) zinc finger protein, which functions as a sequence-specific transcriptional repressor. We generated a fusion protein of the zinc finger domain of Nczf and glutathione S-transferase to identify Nczf-binding consensus DNA sequences with random oligonucleotides of 15 and 35 bases. The consensus binding sequence of core nucleotides contains (A/T/C)CTTT(A/G)TTNT. In a gel mobility shift assay, the probe containing these sequences bound to the fusion protein. In silico analysis, these consensus sequences were found on regulatory regions of the endothelin receptor B and the microphthalmia-associated transcription factor genes, which are involved in neural crest development. These results suggest that Nczf functions as a sequence-specific transcription repressor to regulate neural crest cell development. |
