| First Author | Battu S | Year | 2018 |
| Journal | PLoS Biol | Volume | 16 |
| Issue | 4 | Pages | e2005317 |
| PubMed ID | 29621237 | Mgi Jnum | J:261325 |
| Mgi Id | MGI:6154126 | Doi | 10.1371/journal.pbio.2005317 |
| Citation | Battu S, et al. (2018) Amino acid starvation sensing dampens IL-1beta production by activating riboclustering and autophagy. PLoS Biol 16(4):e2005317 |
| abstractText | Activation of the amino acid starvation response (AAR) increases lifespan and acute stress resistance as well as regulates inflammation. However, the underlying mechanisms remain unclear. Here, we show that activation of AAR pharmacologically by Halofuginone (HF) significantly inhibits production of the proinflammatory cytokine interleukin 1beta (IL-1beta) and provides protection from intestinal inflammation in mice. HF inhibits IL-1beta through general control nonderepressible 2 kinase (GCN2)-dependent activation of the cytoprotective integrated stress response (ISR) pathway, resulting in rerouting of IL-1beta mRNA from translationally active polysomes to inactive ribocluster complexes-such as stress granules (SGs)-via recruitment of RNA-binding proteins (RBPs) T cell-restricted intracellular antigen-1(TIA-1)/TIA-1-related (TIAR), which are further cleared through induction of autophagy. GCN2 ablation resulted in reduced autophagy and SG formation, which is inversely correlated with IL-1beta production. Furthermore, HF diminishes inflammasome activation through suppression of reactive oxygen species (ROS) production. Our study unveils a novel mechanism by which IL-1beta is regulated by AAR and further suggests that administration of HF might offer an effective therapeutic intervention against inflammatory diseases. |
