| First Author | Jain S | Year | 2018 |
| Journal | Sci Rep | Volume | 8 |
| Issue | 1 | Pages | 3386 |
| PubMed ID | 29467543 | Mgi Jnum | J:263083 |
| Mgi Id | MGI:6162859 | Doi | 10.1038/s41598-018-21822-y |
| Citation | Jain S, et al. (2018) AP-2epsilon Expression in Developing Retina: Contributing to the Molecular Diversity of Amacrine Cells. Sci Rep 8(1):3386 |
| abstractText | AP-2 transcription factors play important roles in the regulation of gene expression during development. Four of the five members of the AP-2 family (AP-2alpha, AP-2beta, AP-2gamma and AP-2delta) have previously been shown to be expressed in developing retina. Mouse knockouts have revealed roles for AP-2alpha, AP-2beta and AP-2delta in retinal cell specification and function. Here, we show that the fifth member of the AP-2 family, AP-2epsilon, is also expressed in amacrine cells in developing mammalian and chicken retina. Our data indicate that there are considerably fewer AP-2epsilon-positive cells in the developing mouse retina compared to AP-2alpha, AP-2beta and AP-2gamma-positive cells, suggesting a specialized role for AP-2epsilon in a subset of amacrine cells. AP-2epsilon, which is restricted to the GABAergic amacrine lineage, is most commonly co-expressed with AP-2alpha and AP-2beta, especially at early stages of retinal development. Co-expression of AP-2epsilon and AP-2gamma increases with differentiation. Analysis of previously published Drop-seq data from single retinal cells supports co-expression of multiple AP-2s in the same cell. Since AP-2s bind to their target sequences as either homodimers or heterodimers, our work suggests spatially- and temporally-coordinated roles for combinations of AP-2 transcription factors in amacrine cells during retinal development. |
