| First Author | Zhang S | Year | 2018 |
| Journal | Sci Rep | Volume | 8 |
| Issue | 1 | Pages | 6462 |
| PubMed ID | 29691479 | Mgi Jnum | J:263119 |
| Mgi Id | MGI:6163353 | Doi | 10.1038/s41598-018-24683-7 |
| Citation | Zhang S, et al. (2018) Alternative polyadenylation drives genome-to-phenome information detours in the AMPKalpha1 and AMPKalpha2 knockout mice. Sci Rep 8(1):6462 |
| abstractText | Currently available mouse knockout (KO) lines remain largely uncharacterized for genome-to-phenome (G2P) information flows. Here we test our hypothesis that altered myogenesis seen in AMPKalpha1- and AMPKalpha2-KO mice is caused by use of alternative polyadenylation sites (APSs). AMPKalpha1 and AMPKalpha2 are two alpha subunits of adenosine monophosphate-activated protein kinase (AMPK), which serves as a cellular sensor in regulation of many biological events. A total of 56,483 APSs were derived from gastrocnemius muscles. The differentially expressed APSs (DE-APSs) that were down-regulated tended to be distal. The DE-APSs that were related to reduced and increased muscle mass were down-regulated in AMPKalpha1-KO mice, but up-regulated in AMPKalpha2-KO mice, respectively. Five genes: Car3 (carbonic anhydrase 3), Mylk4 (myosin light chain kinase family, member 4), Neb (nebulin), Obscn (obscurin) and Pfkm (phosphofructokinase, muscle) utilized different APSs with potentially antagonistic effects on muscle function. Overall, gene knockout triggers genome plasticity via use of APSs, completing the G2P processes. However, gene-based analysis failed to reach such a resolution. Therefore, we propose that alternative transcripts are minimal functional units in genomes and the traditional central dogma concept should be now examined under a systems biology approach. |
