| First Author | Schmid A | Year | 2018 |
| Journal | Mol Cell Endocrinol | Volume | 473 |
| Pages | 225-234 | PubMed ID | 29409758 |
| Mgi Jnum | J:265951 | Mgi Id | MGI:6207061 |
| Doi | 10.1016/j.mce.2018.01.023 | Citation | Schmid A, et al. (2018) Regulation of natriuretic peptides postprandially in vivo and of their receptors in adipocytes by fatty acids in vitro. Mol Cell Endocrinol 473:225-234 |
| abstractText | BACKGROUND AND AIM: Natriuretic peptides (NPs) and their receptors gain attention regarding adipocyte function. It was the aim to investigate the expression of natriuretic peptide receptors NPR-A, NPR-B and NPR-C during adipocyte differentiation (AD), upon stimulation with fatty acids (FA), and in murine and human adipose tissue depots (AT) of patients undergoing bariatric surgery (n=44). PATIENTS, MATERIAL AND METHODS: The postprandial regulation of NT-proANP and NT-proBNP levels was measured by ELISA and was studied in two cohorts of healthy individuals undergoing an oral glucose tolerance test (OGTT) (n=100) and an oral lipid tolerance test (OLTT) (n=100). Adipocyte mRNA expression was investigated by quantitative real-time PCR. RESULTS: During AD, an early expression pattern could be described for NPR-C, a bimodal expression for NPR-B and a late expression pattern for NPR-A. NPR-A and NPR-B expression was high in epididymal and subcutaneous AT but low in peri-renal AT of mice. NPR-C showed a differential expression profile. FA stimulation caused a significant and differential regulation of NPRs in adipocytes. Serum NT-proANP and NT-proBNP concentrations did not change during OGTT, whereas NT-proANP significantly declined during OLTT. Basal NT-proANP and NT-proBNP concentrations were positively correlated with each other and with FGF-19 and FGF-21 levels. CONCLUSION: Adipocytes and AT show a characteristic expression of NPRs. FA are able to regulate NPR expression differentially. There is a postprandial and negative regulation of serum NT-proANP concentrations after OLTT and of NPR-A after FA stimulation. Both effects could represent a novel hypothetical negative feedback mechanism on adipocyte lipolysis. |
