| First Author | Kurabayashi N | Year | 2018 |
| Journal | Development | Volume | 145 |
| Issue | 17 | PubMed ID | 30217809 |
| Mgi Jnum | J:266593 | Mgi Id | MGI:6220851 |
| Doi | 10.1242/dev.162529 | Citation | Kurabayashi N, et al. (2018) The LPA-LPA4 axis is required for establishment of bipolar morphology and radial migration of newborn cortical neurons. Development 145(17):dev162529 |
| abstractText | Newborn neurons in the developing neocortex undergo radial migration, a process that is coupled with their precise passage from multipolar to bipolar shape. The cell-extrinsic signals that govern this transition are, however, poorly understood. Here, we find that lysophosphatidic acid (LPA) signaling contributes to the establishment of a bipolar shape in mouse migratory neurons through LPA receptor 4 (LPA4). LPA4 is robustly expressed in migratory neurons. LPA4-depleted neurons show impaired multipolar-to-bipolar transition and become arrested in their migration. Further, LPA4-mediated LPA signaling promotes formation of the pia-directed process in primary neurons overlaid on neocortical slices. In addition, LPA4 depletion is coupled with altered actin organization as well as with destabilization of the F-actin-binding protein filamin A (FlnA). Finally, overexpression of FlnA rescues the morphology and migration defects of LPA4-depleted neurons. Thus, the LPA-LPA4 axis regulates bipolar morphogenesis and radial migration of newborn cortical neurons via remodeling of the actin cytoskeleton. |
