| First Author | Mohammed SG | Year | 2017 |
| Journal | FASEB J | Volume | 31 |
| Issue | 5 | Pages | 1796-1806 |
| PubMed ID | 28100643 | Mgi Jnum | J:267626 |
| Mgi Id | MGI:6267787 | Doi | 10.1096/fj.201600687RRR |
| Citation | Mohammed SG, et al. (2017) Fluid shear stress increases transepithelial transport of Ca(2+) in ciliated distal convoluted and connecting tubule cells. FASEB J 31(5):1796-1806 |
| abstractText | In kidney, transcellular transport of Ca(2+) is mediated by transient receptor potential vanilloid 5 and Na(+)-Ca(2+) exchanger 1 proteins in distal convoluted and connecting tubules (DCTs and CNTs, respectively). It is not yet understood how DCT/CNT cells can adapt to differences in tubular flow rate and, consequently, Ca(2+) load. This study aims to elucidate the molecular mechanisms by which DCT/CNT cells sense fluid dynamics to control transepithelial Ca(2+) reabsorption and whether their primary cilia play an active role in this process. Mouse primary DCT/CNT cultures were subjected to a physiologic fluid shear stress (FSS) of 0.12 dyn/cm(2) Transient receptor potential vanilloid 5 and Na(+)-Ca(2+) exchanger 1 mRNA levels were significantly increased upon FSS exposure compared with static controls. Functional studies with (45)Ca(2+) demonstrated a significant stimulation of transepithelial Ca(2+) transport under FSS compared with static conditions. Primary cilia removal decreased Ca(2+) transport in both static and FSS conditions, a finding that correlated with decreased expression of genes involved in transepithelial Ca(2+) transport; however, FSS-induced stimulation of Ca(2+) transport was still observed. These results indicate that nephron DCT and CNT segments translate FSS into a physiologic response that implicates an increased Ca(2+) reabsorption. Moreover, primary cilia influence transepithelial Ca(2+) transport in DCTs/CNTs, yet this process is not distinctly coupled to FSS sensing by these organelles.-Mohammed, S. G., Arjona, F. J., Latta, F., Bindels, R. J. M., Roepman, R., Hoenderop, J. G. J. Fluid shear stress increases transepithelial transport of Ca(2+) in ciliated distal convoluted and connecting tubule cells. |
