| First Author | Lim HX | Year | 2018 |
| Journal | J Immunol | Volume | 201 |
| Issue | 9 | Pages | 2832-2841 |
| PubMed ID | 30275047 | Mgi Jnum | J:267988 |
| Mgi Id | MGI:6258204 | Doi | 10.4049/jimmunol.1800386 |
| Citation | Lim HX, et al. (2018) Lysyl-Transfer RNA Synthetase Induces the Maturation of Dendritic Cells through MAPK and NF-kappaB Pathways, Strongly Contributing to Enhanced Th1 Cell Responses. J Immunol 201(9):2832-2841 |
| abstractText | In addition to essential roles in protein synthesis, lysyl-tRNA synthetase (KRS) is secreted to trigger a proinflammatory function that induces macrophage activation and TNF-alpha secretion. KRS has been associated with autoimmune diseases such as polymyositis and dermatomyositis. In this study, we investigated the immunomodulatory effects of KRS on bone marrow-derived dendritic cells (DCs) of C57BL/6 mice and subsequent polarization of Th cells and analyzed the underlying mechanisms. KRS-treated DCs increased the expression of cell surface molecules and proinflammatory cytokines associated with DC maturation and activation. Especially, KRS treatment significantly increased production of IL-12, a Th1-polarizing cytokine, in DCs. KRS triggered the nuclear translocation of the NF-kappaB p65 subunit along with the degradation of IkappaB proteins and the phosphorylation of MAPKs in DCs. Additionally, JNK, p38, and ERK inhibitors markedly recovered the degradation of IkappaB proteins, suggesting the involvement of MAPKs as the upstream regulators of NF-kappaB in the KRS-induced DC maturation and activation. Importantly, KRS-treated DCs strongly increased the differentiation of Th1 cells when cocultured with CD4(+) T cells. The addition of anti-IL-12-neutralizing Ab abolished the secretion of IFN-gamma in the coculture, indicating that KRS induces Th1 cell response via DC-derived IL-12. Moreover, KRS enhanced the OVA-specific Th1 cell polarization in vivo following the adoptive transfer of OVA-pulsed DCs. Taken together, these results indicated that KRS effectively induced the maturation and activation of DCs through MAPKs/NF-kappaB-signaling pathways and favored DC-mediated Th1 cell response. |
