| First Author | Wang W | Year | 2018 |
| Journal | Mol Cell Endocrinol | Volume | 477 |
| Pages | 57-69 | PubMed ID | 29870755 |
| Mgi Jnum | J:269756 | Mgi Id | MGI:6270231 |
| Doi | 10.1016/j.mce.2018.06.001 | Citation | Wang W, et al. (2018) C/EBPbeta LIP and c-Jun synergize to regulate expression of the murine progesterone receptor. Mol Cell Endocrinol 477:57-69 |
| abstractText | CCAAT/enhancer binding protein beta (C/EBPbeta) is required for murine mammary ductal morphogenesis and alveologenesis. Progesterone is critical for proliferation and alveologenesis in adult mammary glands, and there is a similar requirement for progesterone receptor isoform B (PRB) in alveologenesis. We examined C/EBPbeta regulation of PR expression. All three C/EBPbeta isoforms, including typically inhibitory LIP, transactivated the PR promoter. LIP, particularly, strongly synergized with c-Jun to drive PR transcription. Endogenous C/EBPbeta and c-Jun stimulated a PR promoter-reporter and these two factors showed promoter occupancy on the endogenous PR gene. Additionally, LIP overexpression elevated endogenous PR protein expression. In pregnancy, both PRB and the relative abundance of LIP among C/EBPbeta isoforms increase. Consistent with a role in PRB expression, in vivo C/EBPbeta and PR isoform A expression showed mutually exclusive localization in mammary epithelium, while C/EBPbeta and PRB largely co-localized. We suggest a critical role for C/EBPbeta, particularly LIP, in PRB expression. |
