| First Author | Matsunaga H | Year | 2018 |
| Journal | Biochem Biophys Res Commun | Volume | 495 |
| Issue | 1 | Pages | 740-748 |
| PubMed ID | 29137982 | Mgi Jnum | J:272370 |
| Mgi Id | MGI:6280118 | Doi | 10.1016/j.bbrc.2017.11.069 |
| Citation | Matsunaga H, et al. (2018) Adipose tissue complement factor B promotes adipocyte maturation. Biochem Biophys Res Commun 495(1):740-748 |
| abstractText | OBJECTIVES: It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism. METHODS: We investigated the expression level of complement system-related genes in adipocytes. To understand the role of CfB in adipocyte, we performed Cfb overexpression in 3T3-L1 preadipocytes and generated adipocyte-specific Cfb transgenic mice. RESULTS: Cfb expression was markedly enhanced in 3T3-L1 adipocytes co-cultured with macrophages following endotoxin stimulation. In Cfb-overexpressing cells, the expression of adipocyte differentiation/maturation-related genes encoding peroxisome proliferator-activated receptor gamma (Ppargamma), adipocyte Protein 2 and perilipin was significantly enhanced. Cfb transgenic mice showed a marked increase in the expression of genes encoding Ppargamma, perilipin, sterol regulatory element-binding protein 1 c, and Cd36 in the subcutaneous adipose tissue. CONCLUSIONS: CfB plays a crucial role in late-phase of adipocyte differentiation and subsequent lipid droplet formation. |
