| First Author | Qin J | Year | 2018 |
| Journal | Biochem Biophys Res Commun | Volume | 500 |
| Issue | 3 | Pages | 597-602 |
| PubMed ID | 29673590 | Mgi Jnum | J:273345 |
| Mgi Id | MGI:6281068 | Doi | 10.1016/j.bbrc.2018.04.110 |
| Citation | Qin J, et al. (2018) The cross roles of sphingosine kinase 1/2 and ceramide glucosyltransferase in cell growth and death. Biochem Biophys Res Commun 500(3):597-602 |
| abstractText | Sphingosine-1-phosphate is synthesized by two sphingosine kinases, cytosolic SK1 and nuclear SK2 but SK2 expression was much higher than SK1in mouse skin fibroblasts. However, in SK2(-/-) cells, SK1 expression was markedly increased to SK2 levels whereas in SK1(-/-) cells, SK2 expression was unaffected. Ceramide, glucosylceramide and sphingosine levels were all increased in SK1(-/-) but less so in SK2(-/-) cells and S1P levels were not significantly reduced in either SK1(-/-) or SK2(-/-) cells. Greatly increased Ceramide glucosyltransferase expression was observed in SK1(-/-) cells but less so in SK2(-/-) cells suggested a role in drug resistance. SK2(-/-) cells grew faster than control and SK1(-/-). The cell division gene PCNA was significantly overexpressed in SK2(-/-) cells, suggesting a cross regulation between SKs and Ceramide glucosyltransferase. |
