| First Author | Inamochi Y | Year | 2016 |
| Journal | Biochem Biophys Rep | Volume | 7 |
| Pages | 150-156 | PubMed ID | 28955901 |
| Mgi Jnum | J:268423 | Mgi Id | MGI:6272556 |
| Doi | 10.1016/j.bbrep.2016.05.016 | Citation | Inamochi Y, et al. (2016) Transcription elongation factor Brd4-P-TEFb accelerates intestinal differentiation-associated SLC2A5 gene expression. Biochem Biophys Rep 7:150-156 |
| abstractText | BACKGROUND: Expression of the fructose transporter gene SLC2A5 and histone acetylation in the transcribed region are induced by differentiation associated-signals such as glucocorticoids and p44/42 mitogen-activated protein kinase (MAPK) inhibition in small intestinal Caco-2 cells. METHODS: We co-treated with glucocorticoid receptor agonist dexamethasone (Dex) and p44/42 MAPK inhibitor PD98059 (PD) in Caco-2 cells with or without Brd4 small hairpin (sh) RNA expression vector, and the cells were analyzed by qRT-PCR and chromatin immunoprecipitation assays. The small intestine of wild-type mice and Brd4(+/-) mice during weaning period were analyzed by qRT-PCR. RESULTS: Co-treatment with Dex and PD increased binding of the bromodomain-containing protein-4 (Brd4)-positive transcriptional elongation factor-b (P-TEFb)-RNA polymerase II complex to acetylated histones in the transcribed region of SLC2A5. Brd4-protein depletion by shRNA revealed that the association of these proteins on the transcribed region of SLC2A5 promoted gene expression in a Brd4-dependent manner. Expression of small-intestine Slc2a5, but not another intestinal gene sucrase-isomaltase, during weaning period, was significantly lower in Brd4(+/-) mice compared with wild-type mice. CONCLUSIONS: Brd4-P-TEFb plays a crucial role in differentiation-associated transcription of SLC2A5 gene in intestinal Caco-2 cells and in the small intestine of mice during weaning period. GENERAL SIGNIFICANCE: Histone acetylation and the transcription elongation factor Brd4 are important for SLC2A5 expression in the small intestine. |
