| First Author | Galván-Peña S | Year | 2019 |
| Journal | Nat Commun | Volume | 10 |
| Issue | 1 | Pages | 338 |
| PubMed ID | 30659183 | Mgi Jnum | J:270154 |
| Mgi Id | MGI:6277232 | Doi | 10.1038/s41467-018-08187-6 |
| Citation | Galvan-Pena S, et al. (2019) Malonylation of GAPDH is an inflammatory signal in macrophages. Nat Commun 10(1):338 |
| abstractText | Macrophages undergo metabolic changes during activation that are coupled to functional responses. The gram negative bacterial product lipopolysaccharide (LPS) is especially potent at driving metabolic reprogramming, enhancing glycolysis and altering the Krebs cycle. Here we describe a role for the citrate-derived metabolite malonyl-CoA in the effect of LPS in macrophages. Malonylation of a wide variety of proteins occurs in response to LPS. We focused on one of these, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In resting macrophages, GAPDH binds to and suppresses translation of several inflammatory mRNAs, including that encoding TNFalpha. Upon LPS stimulation, GAPDH undergoes malonylation on lysine 213, leading to its dissociation from TNFalpha mRNA, promoting translation. We therefore identify for the first time malonylation as a signal, regulating GAPDH mRNA binding to promote inflammation. |
