| First Author | Roby KF | Year | 1995 |
| Journal | Endocrine | Volume | 3 |
| Issue | 8 | Pages | 557-62 |
| PubMed ID | 21153132 | Mgi Jnum | J:28858 |
| Mgi Id | MGI:76390 | Doi | 10.1007/BF02953019 |
| Citation | Roby KF, et al. (1995) Expression and localization of messenger RNA for tumor necrosis factor receptor (TNF-R) i and TNF-RII in pregnant mouse uterus and placenta. Endocrine 3(8):557-562 |
| abstractText | The temporal and cell-specific localization of tumor necrosis factor (TNF) receptor mRNAs in the uterus and placenta during pregnancy in the mouse was investigated. Messenger RNA for TNF and the TNF receptors (TNF-RI, p55/ p60 and TNF-RII, p75/p80) was assessed by northern blot and in situ hybridization. TNF, TNF-RI and TNF-RII specific transcripts were present on days 7 through 18 of pregnancy. Relative concentrations of TNF mRNA decreased from days 7 to 18 with levels being higher in the uterus than the placenta. In contrast TNF-RI mRNA levels were constant throughout gestation and no differences were seen between steady state levels in the uterus and placenta. Two transcripts for TNF-RII (3.6 and 4.5 kb) were identified in all tissues. Steady state levels of TNF-RII mRNA increased throughout gestation and levels were higher in the placenta than in the uterus. On day 9 of gestation, TNF-RI and TNF-RII mRNAs were localized to undecidualized endometrium, mesometrial decidual cells, and the developing placenta. In addition, muscle cells contained TNF-RI but not TNF-RII mRNA. By day 15 of gestation, TNF- RI and TNF-RII transcripts were primarily localized to the uterine epithelium and trophoblast giant cells and spongiotrophoblast cells in the placenta. The results of these studies reveal the uterine and placental cell- specific expression of TNF receptor mRNAs during pregnancy in the mouse and provide insight into the cellular targets of TNF action. |
