| First Author | Gupta M | Year | 1998 |
| Journal | Environ Toxicol Pharmacol | Volume | 5 |
| Issue | 3 | Pages | 205-13 |
| PubMed ID | 21781866 | Mgi Jnum | J:48609 |
| Mgi Id | MGI:1271009 | Doi | 10.1016/s1382-6689(98)00004-0 |
| Citation | Gupta M, et al. (1998) Ah receptor-independent induction of CYP1A2 gene expression in genetically inbred mice. Environ Toxicol Pharmacol 5(3):205-213 |
| abstractText | Acenaphthylene is a tricyclic aromatic hydrocarbon which induces hepatic methoxyresorufin O-demethylase (MROD) activity and CYP1A2 mRNA levels in 2 week-old male B6C3F1 mice. In the present study, this induction response was further investigated in genetically-inbred mice which differ in their aryl-hydrocarbon (Ah)-responsiveness. Acenapthylene (300 mg/kg) induced a 5- to 23-fold induction of MROD activity in Ah-nonresponsive (DBA and SJL) and responsive (C3H, C57/BL6, A/J, CBA and B6C3F1) mice. The highest induction response was observed in the DBA strain in which there was a 23- and 15-fold increase in activity in males and females, respectively. Acenaphthylene also caused a 2-fold increase in CYP1A2 mRNA and immunoreactive protein levels in 2 week-old DBA mice; however, this induction response was not observed in 6 week-old animals. For example, MROD activity in 6 week- old DBA mice was induced < 2-fold by acenaphthylene, mainly as a consequence of increased basal CYP1A2 expression and MROD activity which, at the age of 6 weeks, approached levels induced by acenaphthylene in the 2 week- old mice. This was also observed by immunohistochemical staining with CYP1A2 antibodies of 2 and 6 week-old hepatic tissue from treated and control mice which also showed that CYP1A2 induction was dependent on the age of the animals. (C) 1998 Elsevier Science B.V. All rights reserved. |
