| First Author | Tae HJ | Year | 2017 |
| Journal | Mol Med Rep | Volume | 15 |
| Issue | 6 | Pages | 4049-4054 |
| PubMed ID | 28487987 | Mgi Jnum | J:277021 |
| Mgi Id | MGI:6296518 | Doi | 10.3892/mmr.2017.6526 |
| Citation | Tae HJ, et al. (2017) G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing beta1- and beta2-adrenergic receptors. Mol Med Rep 15(6):4049-4054 |
| abstractText | beta1- and beta2-adrenergic receptors (ARs) regulate cardiac contractility, calcium handling and protein phosphorylation. The present study aimed to examine the expression levels of vascular endothelial growth factor A (VEGFA) and several G proteins, and the phosphorylation of transcription factor GATA binding protein 4 (GATA4), by western blot analysis, using isolated hearts from 6 monthold transgenic (TG) mice that overexpress beta1AR or beta2AR. Cardiac contractility/relaxation and heart rate was increased in both beta1AR TG and beta2AR TG mouse hearts compared with wild type; however, no significant differences were observed between the beta1 and beta2AR TG mouse hearts. Protein expression levels of inhibitory guanine nucleotidebinding protein (Gi) 2, Gi3 and Gproteincoupled receptor kinase 2 were upregulated in both TG mice, although the upregulation of Gi2 was more prominent in the beta2AR TG mice. VEGFA expression levels were also increased in both TG mice, and were highest in the beta1AR TG mice. In addition, the levels of phosphorylatedGATA4 expression were increased in beta1 and beta2AR TG mice. In conclusion, the present study demonstrated that cardiac contractility/relaxation and heart rate is increased in beta1AR TG and beta2AR TG mice, and indicated that this increase may be related to the overexpression of G proteins and Gproteinassociated proteins. |
