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Publication : Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections.

First Author  McGlinn E Year  2019
Journal  Methods Mol Biol Volume  1920
Pages  183-218 PubMed ID  30737693
Mgi Jnum  J:274906 Mgi Id  MGI:6296898
Doi  10.1007/978-1-4939-9009-2_12 Citation  McGlinn E, et al. (2019) Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections. Methods Mol Biol 1920:183-218
abstractText  Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.
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