First Author | McGlinn E | Year | 2019 |
Journal | Methods Mol Biol | Volume | 1920 |
Pages | 183-218 | PubMed ID | 30737693 |
Mgi Jnum | J:274906 | Mgi Id | MGI:6296898 |
Doi | 10.1007/978-1-4939-9009-2_12 | Citation | McGlinn E, et al. (2019) Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections. Methods Mol Biol 1920:183-218 |
abstractText | Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here. |