| First Author | Joachimsthaler A | Year | 2019 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 60 |
| Issue | 6 | Pages | 2152-2164 |
| PubMed ID | 31100107 | Mgi Jnum | J:276024 |
| Mgi Id | MGI:6304741 | Doi | 10.1167/iovs.18-26356 |
| Citation | Joachimsthaler A, et al. (2019) Mouse Cones Adapt Fast, Rods Slowly In Vivo. Invest Ophthalmol Vis Sci 60(6):2152-2164 |
| abstractText | Purpose: To study rod- and cone-driven adaptation dynamics separately, we used the silent substitution technique to selectively stimulate rods or cones in the Opn1lwLIAIS (LIAIS) mouse, in which the native M-cone pigment is replaced by a human L-cone pigment (L*). Methods: ERG recordings were performed on anesthetized LIAIS mice. ERG stimuli were sinusoidally modulated. After 10 minutes of adaptation to 0.4 candela per square meter (cd/m2) ERGs were measured, followed by 11-minute adaptation to 8.8 cd/m2 background and recordings directly after the luminance increase and every second minute. Finally, during adaptation to 0.4 cd/m2 for 32 minutes, ERG responses were recorded directly after the change in background and every second minute. This protocol was repeated with rod-isolating stimuli (8 Hz; 75% rod contrast), L*-cone-isolating stimuli (12 Hz; 55% cone contrast) and white light (8 Hz and 12 Hz; 100% Michelson contrast). Results: At 8.8 cd/m2, responses directly displayed photopic response properties without further changes in either cone or white light responses. Rod-driven responses were very small. After the return to 0.4 cd/m2, both rod-driven and white light responses increased over a time course of about 30 minutes. Cone-driven responses were very small. Response phases changed directly after a change in background without further alterations. Conclusions: Rod- and cone-driven signal pathways display strongly different adaptation characteristics: adaptation of cone-driven responses to photopic conditions is very fast, whereas rod-driven responses change with a time course up to 30 minutes during scotopic conditions. Luminance responses are cone-driven at 8.8 cd/m2 and rod-driven at 0.4 cd/m2. |
