| First Author | Pijuan-Sala B | Year | 2020 |
| Journal | Nat Cell Biol | Volume | 22 |
| Issue | 4 | Pages | 487-497 |
| PubMed ID | 32231307 | Mgi Jnum | J:291166 |
| Mgi Id | MGI:6442747 | Doi | 10.1038/s41556-020-0489-9 |
| Citation | Pijuan-Sala B, et al. (2020) Single-cell chromatin accessibility maps reveal regulatory programs driving early mouse organogenesis. Nat Cell Biol 22(4):487-497 |
| abstractText | During mouse embryonic development, pluripotent cells rapidly divide and diversify, yet the regulatory programs that define the cell repertoire for each organ remain ill-defined. To delineate comprehensive chromatin landscapes during early organogenesis, we mapped chromatin accessibility in 19,453 single nuclei from mouse embryos at 8.25 days post-fertilization. Identification of cell-type-specific regions of open chromatin pinpointed two TAL1-bound endothelial enhancers, which we validated using transgenic mouse assays. Integrated gene expression and transcription factor motif enrichment analyses highlighted cell-type-specific transcriptional regulators. Subsequent in vivo experiments in zebrafish revealed a role for the ETS factor FEV in endothelial identity downstream of ETV2 (Etsrp in zebrafish). Concerted in vivo validation experiments in mouse and zebrafish thus illustrate how single-cell open chromatin maps, representative of a mammalian embryo, provide access to the regulatory blueprint for mammalian organogenesis. |
