| First Author | Tanaka S | Year | 2019 |
| Journal | Int J Mol Med | Volume | 43 |
| Issue | 5 | Pages | 2164-2176 |
| PubMed ID | 30896835 | Mgi Jnum | J:290309 |
| Mgi Id | MGI:6442312 | Doi | 10.3892/ijmm.2019.4143 |
| Citation | Tanaka S, et al. (2019) Involvement of PLAGL1/ZAC1 in hypocretin/orexin transcription. Int J Mol Med 43(5):2164-2176 |
| abstractText | The hypocretin/orexin neuropeptide system coordinates the regulation of various physiological processes. Our previous study reported that a reduction in the expression of pleomorphic adenoma genelike 1 (Plagl1), which encodes a C2H2 zincfinger transcription factor, occurs in hypocretin neuronablated transgenic mice, suggesting that PLAGL1 is coexpressed in hypocretin neurons and regulates hypocretin transcription. The present study examined whether canonical preprohypocretin transcription is functionally modulated by PLAGL1. Double immunostaining indicated that the majority of hypocretin neurons were positive for PLAGL1 immunoreactivity in the nucleus. Notably, PLAGL1 immunoreactivity in hypocretin neurons was altered in response to several conditions affecting hypocretin function. An uneven localization of PLAGL1 was detected in the nuclei of hypocretin neurons following sleep deprivation. Chromatin immunoprecipitation revealed that endogenous PLAGL1 may bind to a putative PLAGL1binding site in the proximal region of the hypocretin gene, in the murine hypothalamus. In addition, electroporation of the PLAGL1 expression vector into the fetal hypothalamus promoted hypothalamic hypocretin transcription. These results suggested that PLAGL1 may regulate hypothalamic hypocretin transcription. |
