| First Author | Takagi A | Year | 2020 |
| Journal | Gene Expr Patterns | Volume | 35 |
| Pages | 119094 | PubMed ID | 31899345 |
| Mgi Jnum | J:300114 | Mgi Id | MGI:6392139 |
| Doi | 10.1016/j.gep.2019.119094 | Citation | Takagi A, et al. (2019) Dynamic Delta-like1 expression in presomitic mesoderm cells during somite segmentation. Gene Expr Patterns 35:119094 |
| abstractText | During somite segmentation, the expression of clock genes such as Hes7 oscillates synchronously in the presomitic mesoderm (PSM). This synchronous oscillation slows down in the anterior PSM, leading to wave-like propagating patterns from the posterior to anterior PSM. Such dynamic expression depends on Notch signaling and is critical for somite formation. However, it remains to be determined how slowing oscillations in the anterior PSM are controlled, and whether the expression of the Notch ligand Delta-like1 (Dll1) oscillates on the surface of individual PSM cells, as postulated to be responsible for synchronous oscillation. Here, by using Dll1 fluorescent reporter mice, we performed live-imaging of Dll1 expression in PSM cells and found the oscillatory expression of Dll1 protein on the cell surface regions. Furthermore, a comparison of live-imaging of Dll1 and Hes7 oscillations revealed that the delay from Dll1 peaks to Hes7 peaks increased in the anterior PSM, suggesting that the Hes7 response to Dll1 becomes slower in the anterior PSM. These results raise the possibility that Dll1 protein oscillations on the cell surface regulate synchronous Hes7 oscillations, and that the slower response of Hes7 to Dll1 leads to slower oscillations in the anterior PSM. |
