| First Author | Ikeda A | Year | 2019 |
| Journal | Commun Biol | Volume | 2 |
| Pages | 371 | PubMed ID | 31633062 |
| Mgi Jnum | J:289523 | Mgi Id | MGI:6433684 |
| Doi | 10.1038/s42003-019-0627-8 | Citation | Ikeda A, et al. (2019) High-fidelity endonuclease variant HypaCas9 facilitates accurate allele-specific gene modification in mouse zygotes. Commun Biol 2:371 |
| abstractText | CRISPR/Cas9 has been widely used for the efficient generation of genetically modified animals; however, this system could have unexpected off-target effects. In the present study, we confirmed the validity of a high-fidelity Cas9 variant, HypaCas9, for accurate genome editing in mouse zygotes. HypaCas9 efficiently modified the target locus while minimizing off-target effects even in a single-nucleotide mismatched sequence. Furthermore, by applying HypaCas9 to the discrimination of SNP in hybrid strain-derived zygotes, we accomplished allele-specific gene modifications and successfully generated mice with a monoallelic mutation in an essential gene. These results suggest that the improved accuracy of HypaCas9 facilitates the generation of genetically modified animals. |
