| First Author | Xia D | Year | 2020 |
| Journal | Mol Immunol | Volume | 117 |
| Pages | 168-179 | PubMed ID | 31812793 |
| Mgi Jnum | J:289963 | Mgi Id | MGI:6435254 |
| Doi | 10.1016/j.molimm.2019.10.009 | Citation | Xia D, et al. (2020) LncRNA NEAT1 reversed the hindering effects of miR-495-3p/STAT3 axis and miR-211/PI3K/AKT axis on sepsis-relevant inflammation. Mol Immunol 117:168-179 |
| abstractText | OBJECTIVE: This investigation was intended to elucidate lncRNA-miRNA networks that could explain inflammation underlying sepsis progression. METHODS: In the first place, four kinds of mice models were established, namely, SHAM group (n=30), trauma (TH) group (n=30), lipopolysaccharide (LPS) group (n=30) and TH+LPS group (n=30). Their lung, spleen and liver tissues were gathered for determination of TNF-alpha, IL-6, IL-10 and MCP-1 levels. Furthermore, mouse mononuclear macrophage leukemia cell line (RAW264.7) was stimulated by LPS to establish inflammation cell models. Then si-NEAT1s, pcDNA3.1-NEAT1, miR-495-3p mimic, miR-495-3p inhibitor, miR-NC, miR-211 mimic and miR-211 inhibitor were, respectively, transfected into the cells, so as to observe the impacts of NEAT1, miR-495-3p and miR-211 on cytokine levels released by the cells. RESULTS: The survival condition of mice in the TH+LPS group was undesirable, in relative to mice in the LPS group and SHAM group (both P<0.05). High-level NEAT1 and low-level miR-495-3p/miR-211 were associated with poor survival of mice in the TH+LPS group (P<0.05). Additionally, the correlation between NEAT1/miR-495-3p/miR-211 level and cytokine level was the strongest among TH+LPS-treated mice, in comparison to mice treated by TH or LPS. Furthermore, up-regulation of NEAT1 level and down-regulation of miR-495-3p/miR-211 level could aggravate inflammation in LPS-treated RAW264.7 cells. The miR-495-3p and miR-211 herein, were both subjected to sponging of NEAT1, possibly affected inflammation responses in RAW264.7 cells, respectively, through modulating STAT3 and PI3K/AKT signaling. CONCLUSION: LncRNA NEAT1 exhibited great potential sepsis diagnosis and treatment, considering its modifying miR-495-3p/STAT3 axis and miR-211/PI3K/AKT axis in inflammation cell models. |
