| First Author | Sagoshi S | Year | 2020 |
| Journal | Neuroscience | Volume | 438 |
| Pages | 182-197 | PubMed ID | 32387645 |
| Mgi Jnum | J:293152 | Mgi Id | MGI:6445765 |
| Doi | 10.1016/j.neuroscience.2020.04.047 | Citation | Sagoshi S, et al. (2020) Detection and Characterization of Estrogen Receptor Beta Expression in the Brain with Newly Developed Transgenic Mice. Neuroscience 438:182-197 |
| abstractText | Two types of nuclear estrogen receptors, ERalpha and ERbeta, have been shown to be differentially involved in the regulation of various types of behaviors. Due to a lack of tools for identifying ERbeta expression, detailed anatomical distribution and neurochemical characteristics of ERbeta expressing cells and cellular co-expression with ERalpha remain unclear. We have generated transgenic mice ERbeta-RFP(tg), in which RFP was inserted downstream of ERbeta BAC promotor. We verified RFP signals as ERbeta by confirming: (1) high ERbeta mRNA levels in RFP-expressing cells collected by fluorescence-activated cell sorting; and (2) co-localization of ERbeta mRNA and RFP proteins in the paraventricular nucleus (PVN). Strong ERbeta-RFP signals were found in the PVN, medial preoptic area (MPOA), bed nucleus of the stria terminalis, medial amygdala (MeA), and dorsal raphe nucleus (DRN). In the MPOA and MeA, three types of cell populations were identified; those expressing both ERalpha and ERbeta, and those expressing exclusively either ERalpha or ERbeta. The majority of PVN and DRN cells expressed only ERbeta-RFP. Further, ERbeta-RFP positive cells co-expressed oxytocin in the PVN, and tryptophan hydroxylase 2 and progesterone receptors in the DRN. In the MeA, some ERbeta-RFP positive cells co-expressed oxytocin receptors. These findings collectively suggest that ERbeta-RFP(tg) mice can be a powerful tool for future studies on ERbeta function in the estrogenic regulation of social behaviors. |
