| First Author | Houliston E | Year | 1988 |
| Journal | Development | Volume | 102 |
| Issue | 2 | Pages | 271-8 |
| PubMed ID | 3046911 | Mgi Jnum | J:289360 |
| Mgi Id | MGI:6436739 | Doi | 10.1242/dev.102.2.271 |
| Citation | Houliston E, et al. (1988) Expression of nuclear lamins during mouse preimplantation development. Development 102(2):271-8 |
| abstractText | The expression of nuclear lamins during mouse preimplantation development was studied by immunofluorescence, immunoblotting and immunoprecipitation. Two sera were used, specific either for lamin B or lamins A and C. Both sera gave a positive staining of the nuclear periphery throughout preimplantation development (fertilized eggs to late blastocysts). Immunoblots revealed that the three lamins were present in eggs and blastocysts. However, lamin A from eggs was found to have a higher apparent Mr than lamin A from blastocysts and other mouse cells. Using immunoprecipitation, synthesis of lamin A was detected in eggs while synthesis of lamin B was detected in 8-cell embryos and blastocysts, indicating that at least some of the lamins used during early development do not come from a store in the egg. These results are discussed in relation to the possible role of lamins during cell differentiation. |
