| First Author | Liu G | Year | 2019 |
| Journal | Mol Reprod Dev | Volume | 86 |
| Issue | 1 | Pages | 20-31 |
| PubMed ID | 30358927 | Mgi Jnum | J:289364 |
| Mgi Id | MGI:6436759 | Doi | 10.1002/mrd.23078 |
| Citation | Liu G, et al. (2019) Primordial follicle activation is affected by the absence of Sohlh1 in mice. Mol Reprod Dev 86(1):20-31 |
| abstractText | Previous studies have reported that only primordial follicles and empty follicles can be found in 7.5 days postparturition (dpp) Sohlh1(-/-) mouse ovaries and females are infertility. There appears to be a defect in follicle development during the primordial-to-primary follicle transition in Sohlh1(-/-) mouse ovaries. However, detailed analyses of these phenomena have not been performed. In this study, we used Sohlh1(-/-) transgenic mice to explore the role of Sohlh1 in folliculogenesis. The results showed that only primordial follicles and empty follicles can be observed in Sohlh1(-/-) ovaries from 0.5 to 23.5 dpp. The expression of Foxo3 and FOXO3 was downregulated; nucleocytoplasmic shuttling of FOXO3 was normal in 7.5-dpp Sohlh1(+/+) but not Sohlh1(-/-) ovaries; and primordial follicle activation (PFA) was not observed in 7.5-dpp Sohlh1(-/-) mice. The expression levels of KIT, AKT, and P308-AKT were downregulated (p < 0.05), whereas that of P473-AKT was not significantly changed (p > 0.05). The KIT/PI3K/AKT pathway was inhibited. Furthermore, we conducted a dual luciferase assay and chromatin immunoprecipitation. The results showed that SOHLH1 can upregulate the Kit gene by binding to the -3698 bp E-box motif. The absence of Sohlh1 may affect PFA in mouse ovaries via downregulation of Kit and inhibition of the KIT/PI3K/AKT pathway. |
