| First Author | Nakamura K | Year | 2019 |
| Journal | J Physiol Sci | Volume | 69 |
| Issue | 3 | Pages | 453-463 |
| PubMed ID | 30758780 | Mgi Jnum | J:295774 |
| Mgi Id | MGI:6454387 | Doi | 10.1007/s12576-018-00654-5 |
| Citation | Nakamura K, et al. (2019) Overexpression of neuronal K(+)-Cl(-) co-transporter enhances dendritic spine plasticity and motor learning. J Physiol Sci 69(3):453-463 |
| abstractText | The neuronal K(+)-Cl(-) cotransporter KCC2 maintains a low intracellular Cl(-) concentration and facilitates hyperpolarizing GABAA receptor responses. KCC2 also plays a separate role in stabilizing and enhancing dendritic spines in the developing nervous system. Using a conditional transgenic mouse strategy, we examined whether overexpression of KCC2 enhances dendritic spines in the adult nervous system and characterized the effects on spine dynamics in the motor cortex in vivo during rotarod training. Mice overexpressing KCC2 showed significantly increased spine density in the apical dendrites of layer V pyramidal neurons, measured in vivo using two-photon imaging. During modest accelerated rotarod training, mice overexpressing KCC2 displayed enhanced spine formation rates, greater balancing skill at higher rotarod speeds and a faster rate of learning in this ability. Our results demonstrate that KCC2 enhances spine density and dynamics in the adult nervous system and suggest that KCC2 may play a role in experience-dependent synaptic plasticity. |
