| First Author | Liu Y | Year | 2019 |
| Journal | J Int Med Res | Volume | 47 |
| Issue | 6 | Pages | 2499-2506 |
| PubMed ID | 31096818 | Mgi Jnum | J:295813 |
| Mgi Id | MGI:6454524 | Doi | 10.1177/0300060519847880 |
| Citation | Liu Y, et al. (2019) Expression levels of cathepsin L and cystatin C in a hyperglycemic environment were associated with aortic aneurysm development in a mouse model. J Int Med Res 47(6):2499-2506 |
| abstractText | OBJECTIVES: Diabetes mellitus (DM) attenuates the development of aortic aneurysms (AA). This study investigated the expression of cathepsin L and cystatin C in a hyperglycemic environment, and the influence of these proteins on AA development. METHODS: Mice were divided into AA and DM+AA groups ( n=30 per group). DM was induced by injection of streptozotocin; AA was induced by injection of angiotensin II. Doppler examination was used to measure aortic diameter, and Weigert's elastic stain was used to detect elastin degradation. Cathepsin L and cystatin C in aortic tissue were examined by western blotting, immunohistochemistry, and polymerase chain reaction. RESULTS: Aortic diameter in the DM+AA group was less than that in the AA group, and elastin fragmentation grade of the aortic wall was reduced in the DM+AA group. More cathepsin L-positive cells were observed in the AA group than in the DM+AA group; conversely, more cystatin C-positive cells were observed in the DM+AA group than in the AA group. Both protein and mRNA levels of cathepsin L and cystatin C showed similar trends to those observed in immunohistochemistry. CONCLUSIONS: Expression levels of cathepsin L and cystatin C in a hyperglycemic environment were associated with AA development in a mouse model. |
