| First Author | Knutsen LE | Year | 2019 |
| Journal | Immunogenetics | Volume | 71 |
| Issue | 4 | Pages | 321-333 |
| PubMed ID | 30535636 | Mgi Jnum | J:295107 |
| Mgi Id | MGI:6459649 | Doi | 10.1007/s00251-018-01100-x |
| Citation | Knutsen LE, et al. (2019) Evidence of functional Cd94 polymorphism in a free-living house mouse population. Immunogenetics 71(4):321-333 |
| abstractText | The CD94 receptor, expressed on natural killer (NK) and CD8+ T cells, is known as a relatively non-polymorphic receptor with orthologues in humans, other primates, cattle, and rodents. In the house mouse (Mus musculus), a single allele is highly conserved among laboratory strains, and reports of allelic variation in lab- or wild-living mice are lacking, except for deficiency in one lab strain (DBA/2J). The non-classical MHC-I molecule Qa-1b is the ligand for mouse CD94/NKG2A, presenting alternative non-americ fragment of leader peptides (Qa-1 determinant modifier (Qdm)) from classical MHC-I molecules. Here, we report a novel allele identified in free-living house mice captured in Norway, living among individuals carrying the canonical Cd94 allele. The novel Cd94(LocA) allele encodes 12 amino acid substitutions in the extracellular lectin-like domain. Flow cytometric analysis of primary NK cells and transfected cells indicates that the substitutions prevent binding of CD94 mAb and Qa-1b/Qdm tetramers. Our data further indicate correlation of Cd94 polymorphism with the two major subspecies of house mice in Europe. Together, these findings suggest that the Cd94(LocA)/NKG2A heterodimeric receptor is widely expressed among M. musculus subspecies musculus, with ligand-binding properties different from mice of subspecies domesticus, such as the C57BL/6 strain. |
