| First Author | Barnes AP | Year | 2004 |
| Journal | Brain Res Mol Brain Res | Volume | 124 |
| Issue | 2 | Pages | 105-13 |
| PubMed ID | 15135218 | Mgi Jnum | J:296187 |
| Mgi Id | MGI:6468062 | Doi | 10.1016/j.molbrainres.2003.12.020 |
| Citation | Barnes AP, et al. (2004) The identification of a second actin-binding region in spinophilin/neurabin II. Brain Res Mol Brain Res 124(2):105-13 |
| abstractText | Spinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons. Previously, the actin-binding domain (ABD) of spinophilin was localized to a domain between amino acids (aa) 1 and 154. In a mass spectrometry screen for spinophilin-binding proteins, we have identified an additional actin-binding region between aa 151 and 282. F-actin co-sedimentation and GST affinity chromotography experiments further substantiate this result. Phalloidin staining of Rat2 fibroblasts transiently expressing GFP-spinophilin deletion constructs indicates co-localization with a subset of actin. Regions of spinophilin that lack the revised ABD (aa 1-230) do not co-localize with phalloidin-labeled actin, suggesting that the actin-binding domain contributes to directing the subcellular distribution of spinophilin. Targeting experiments using primary hippocampal cultures indicate that only the first actin-binding site contributes to dendritic spine localization. The second ABD targets to spines inefficiently and thus may interact with and affect actin filaments in a different manner than the first ABD. |
