| First Author | Mukai K | Year | 2021 |
| Journal | Nat Commun | Volume | 12 |
| Issue | 1 | Pages | 61 |
| PubMed ID | 33397928 | Mgi Jnum | J:300956 |
| Mgi Id | MGI:6504648 | Doi | 10.1038/s41467-020-20234-9 |
| Citation | Mukai K, et al. (2021) Homeostatic regulation of STING by retrograde membrane traffic to the ER. Nat Commun 12(1):61 |
| abstractText | Coat protein complex I (COP-I) mediates the retrograde transport from the Golgi apparatus to the endoplasmic reticulum (ER). Mutation of the COPA gene, encoding one of the COP-I subunits (alpha-COP), causes an immune dysregulatory disease known as COPA syndrome. The molecular mechanism by which the impaired retrograde transport results in autoinflammation remains poorly understood. Here we report that STING, an innate immunity protein, is a cargo of the retrograde membrane transport. In the presence of the disease-causative alpha-COP variants, STING cannot be retrieved back to the ER from the Golgi. The forced Golgi residency of STING results in the cGAS-independent and palmitoylation-dependent activation of the STING downstream signaling pathway. Surf4, a protein that circulates between the ER/ ER-Golgi intermediate compartment/ Golgi, binds STING and alpha-COP, and mediates the retrograde transport of STING to the ER. The STING/Surf4/alpha-COP complex is disrupted in the presence of the disease-causative alpha-COP variant. We also find that the STING ligand cGAMP impairs the formation of the STING/Surf4/alpha-COP complex. Our results suggest a homeostatic regulation of STING at the resting state by retrograde membrane traffic and provide insights into the pathogenesis of COPA syndrome. |
