| First Author | Patel A | Year | 2020 |
| Journal | Development | Volume | 147 |
| Issue | 24 | PubMed ID | 33158926 |
| Mgi Jnum | J:357074 | Mgi Id | MGI:6505234 |
| Doi | 10.1242/dev.193649 | Citation | Patel A, et al. (2020) A molecular and cellular analysis of human embryonic optic fissure closure related to the eye malformation coloboma. Development 147(24) |
| abstractText | Ocular coloboma is a congenital eye malformation, resulting from a failure in optic fissure closure (OFC) and causing visual impairment. There has been little study of the epithelial fusion process underlying closure in the human embryo and coloboma aetiology remains poorly understood. We performed RNAseq of cell populations isolated using laser capture microdissection to identify novel human OFC signature genes and probe the expression profile of known coloboma genes, along with a comparative murine analysis. Gene set enrichment patterns showed conservation between species. Expression of genes involved in epithelial-to-mesenchymal transition was transiently enriched in the human fissure margins during OFC at days 41-44. Electron microscopy and histological analyses showed that cells transiently delaminate at the point of closure, and produce cytoplasmic protrusions, before rearranging to form two continuous epithelial layers. Apoptosis was not observed in the human fissure margins. These analyses support a model of human OFC in which epithelial cells at the fissure margins undergo a transient epithelial-to-mesenchymal-like transition, facilitating cell rearrangement to form a complete optic cup. |
