| First Author | Wang Y | Year | 2020 |
| Journal | Biochem Biophys Res Commun | Volume | 527 |
| Issue | 1 | Pages | 8-14 |
| PubMed ID | 32446395 | Mgi Jnum | J:302654 |
| Mgi Id | MGI:6509387 | Doi | 10.1016/j.bbrc.2020.04.078 |
| Citation | Wang Y, et al. (2020) CENP-W regulates kinetochore-microtubule attachment and meiotic progression of mouse oocytes. Biochem Biophys Res Commun 527(1):8-14 |
| abstractText | Oocyte meiotic maturation failure and unfaithful chromosome segregation are major causes for female infertility. Here, we showed that CENP-W, a relatively novel member of the kinetochore protein family, was expressed in mouse oocytes from the germinal vesicle (GV) to metaphase II (MII) stages. Confocal microscopy revealed that CENP-W was localized in the germinal vesicle in the GV stage, and then became concentrated on kinetochores during oocyte maturation. Knockdown of CENP-W by specific siRNA injection in vitro caused kinetochore-microtubule detachment, resulting in severely defective spindles and misaligned chromosomes, leading to metaphase I arrest and failure of first polar body (PB1) extrusion. Correspondingly, spindle assembly checkpoint (SAC) activation was observed in CENP-W knockdown oocytes even after 10h of culture. Our results suggest that CENP-W acts as a kinetochore protein, which takes part in kinetochore-microtubule attachment, thus mediating the progression of oocyte meiotic maturation. |
