| First Author | Feng M | Year | 2013 |
| Journal | J Surg Res | Volume | 180 |
| Issue | 1 | Pages | 156-61 |
| PubMed ID | 23157925 | Mgi Jnum | J:302750 |
| Mgi Id | MGI:6509691 | Doi | 10.1016/j.jss.2012.09.042 |
| Citation | Feng M, et al. (2013) Matrix metalloprotease 9 promotes liver recovery from ischemia and reperfusion injury. J Surg Res 180(1):156-61 |
| abstractText | BACKGROUND: Matrix metalloprotease (MMP) 9 has been always considered as a destructor of extracellular matrix, promoting liver injury and metastasis of carcinoma. In this study, we investigated the role of MMP-9 in liver wound healing from ischemia and reperfusion injury (IRI). METHODS: MMP9-/- mice were used to establish partial hepatic IRI model. Serum alanine aminotransferase and hepatic cytokines (tumor necrosis factor alpha, interleukin [IL]-1beta, IL-10, and transforming growth factor beta [TGF-beta]) levels were analyzed after IRI. Hepatic stellate cells were isolated from wild-type mice to determine the effect of MMP-9 on TGF-beta activation. In addition, the effect of TGF-beta on liver wound healing from IRI was determined. RESULTS: Liver recovery from IRI was impaired in MMP9-/- mice, which was described as elevated serum alanine aminotransferase, hepatic tumor necrosis factor alpha, and IL-1beta levels. Meanwhile, TGF-beta-active protein level was decreased in the liver of MMP9-/- mice. In vitro test, the activation of TGF-beta was suppressed in the presence of anti-MMP-9 monoclonal antibody. TGF-beta treatment promoted liver recovery from IRI in MMP9-/- mice. CONCLUSIONS: MMP-9 promoted liver recovery from IRI by activating TGF-beta. Thus, MMP-9 plays dual roles (bad and good) in liver IRI, depending on the timing. |
