| First Author | Qazi TJ | Year | 2021 |
| Journal | Neurosci Lett | Volume | 740 |
| Pages | 135465 | PubMed ID | 33166640 |
| Mgi Jnum | J:304540 | Mgi Id | MGI:6509870 |
| Doi | 10.1016/j.neulet.2020.135465 | Citation | Qazi TJ, et al. (2021) Upregulation of mir-132 induces dopaminergic neuronal death via activating SIRT1/P53 pathway. Neurosci Lett 740:135465 |
| abstractText | For several neurodegenerative disorders, including Parkinson's Disease (PD) and Alzheimer's Disease (AD), microRNAs (miRNAs) have been known to play a crucial role. So, in this study miR-132 and its role in PD cell models was investigated. We wanted to investigate the survival or death pathway involved in PD. We observed the expression levels of miR-132 in MPP+ - treated SH-SY5Y cell line, which acted as a PD cell model, and found an increased expression of miR-132. Moreover, through the Dual-Luciferase(R) Reporter (DLR) Assay, it was also revealed that miR-132 targets SIRT1 3'UTR, a histone deacetylase, and decreases its activity, which results in increased acetylation of p53, an apoptotic inducer. p53 acetylation leads to overexpression of other pro-apoptotic genes like Puma and Noxa, which eventually leads to cell death. Here, we show that the upregulation of miR-132 in SH-SY5Y cells can induce apoptosis through the SIRT1/p53 pathway. |
