| First Author | Liu Y | Year | 2020 |
| Journal | Cell | Volume | 183 |
| Issue | 6 | Pages | 1665-1681.e18 |
| PubMed ID | 33188776 | Mgi Jnum | J:299738 |
| Mgi Id | MGI:6490145 | Doi | 10.1016/j.cell.2020.10.026 |
| Citation | Liu Y, et al. (2020) High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue. Cell 183(6):1665-1681.e18 |
| abstractText | We present deterministic barcoding in tissue for spatial omics sequencing (DBiT-seq) for co-mapping of mRNAs and proteins in a formaldehyde-fixed tissue slide via next-generation sequencing (NGS). Parallel microfluidic channels were used to deliver DNA barcodes to the surface of a tissue slide, and crossflow of two sets of barcodes, A1-50 and B1-50, followed by ligation in situ, yielded a 2D mosaic of tissue pixels, each containing a unique full barcode AB. Application to mouse embryos revealed major tissue types in early organogenesis as well as fine features like microvasculature in a brain and pigmented epithelium in an eye field. Gene expression profiles in 10-mum pixels conformed into the clusters of single-cell transcriptomes, allowing for rapid identification of cell types and spatial distributions. DBiT-seq can be adopted by researchers with no experience in microfluidics and may find applications in a range of fields including developmental biology, cancer biology, neuroscience, and clinical pathology. |
