| First Author | Peng Y | Year | 2021 |
| Journal | Am J Physiol Cell Physiol | PubMed ID | 33826407 |
| Mgi Jnum | J:304784 | Mgi Id | MGI:6695017 |
| Doi | 10.1152/ajpcell.00578.2020 | Citation | Peng Y, et al. (2021) MicroRNA-129-5p Inhibited C2C12 Myogenesis and Repressed Slow Fiber Gene Expression in vitro. Am J Physiol Cell Physiol |
| abstractText | The miR-129 family is widely reported as tumor repressors, while, their roles in skeletal muscle have not been fully investigated. Here, the function and mechanism of miR-129-5p in skeletal muscle, a member of the miR-129 family, were explored using C2C12 cell line. Our study shown that miR-129-5p was widely detected in mouse tissues, with the highest expression in skeletal muscle. Gain- and loss-of-function study shown that miR-129-5p could negatively regulate myogenic differentiation, indicated by reduced ratio of MyHC-positive myofibers and repressed expression of myogenic genes, such as MyoD, MyoG and MyHC. Furthermore, miR-129-5p was more enriched in fast extensor digitalis lateralis (EDL) than in slow soleus (SOL). Enhanced miR-129-5p could significantly reduce the expression of mitochondrial cox family, together with that of MyHC I, and knockdown of miR-129-5p conversely increased the expression of cox genes and MyHC I. Mechanistically, miR-129-5p directly targeted the 3'-UTR of Mef2a, which was suppressed by miR-129-5p agomir at both mRNA and protein levels in C2C12 cells. Moreover, overexpression of Mef2a could rescue the inhibitory effects of miR-129-5p on the expression of myogenic factors and MyHC I. Collectively, our data revealed that miR-129-5p as a negative regulator of myogenic differentiation and slow fiber gene expression, thus affecting body metabolic homeostasis. |
